Isha Diagnostic Centre started as Isha Laboratory in the year 1997 at Guruwar Peth which exists till date. In the year 2001 Isha Laboratory opened its branch at Sadashiv Peth, Laxmi Road. Both the laboratories provided Pathology services to the patient. The Laxmi Road branch provided extra services such as ECG and Pre-operative fitness. In the year 2013 Isha Laboratory Laxmi Road branch was changed to Isha Diagnostic Centre since it expanded and provided pathology services such as Sonography, Color Doppler, Digital X-ray along with the preexisting services.